【文獻標(biāo)題】Enantioselective toxicity ａnd oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al
【作者單位】中國農(nóng)業(yè)科學(xué)院煙草研究所（Tobacco Research Institute of Chinese Academy of Agricultural Sciences）
【文獻中引用產(chǎn)品】
蚯蚓8-羥基脫氧鳥苷（8-OHdG）ELISA試劑盒
蚯蚓細胞色素P450(CYP450)ELISA試劑盒
蚯蚓羥基自由基(OH)ELISA檢測試劑盒
【關(guān)鍵詞】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic
【DOI】https://doi.org/10.1016/j.scitotenv.2020.142630
【影響因子(IF)】8.6
【出版期刊】《Science of the Total Environment》
【產(chǎn)品原文引用】
The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, ａnd activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle ａnd method of 8-OhdG ａnd CYP450 are the same as those of OH,
完整版PDF文獻請咨詢在線客服或者電話聯(lián)系我司業(yè)務(wù)員！
更多公司福利請關(guān)注“恒遠生物”微信公眾號！
或訪問恒遠生物官網(wǎng)（http://www.hnybio.com）
原創(chuàng)作者：上海恒遠生物科技有限公司
總訪問量：7702616 管理登錄
