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【文獻(xiàn)標(biāo)題】Nitrogen Metabolism in Acorus calamus L. Leaves Induced Changes in Response to Microcystin–LR at Environmentally Relevant Concentrations
【作者】Guoyuan Chen,Qingsong Li,Mingxian Bai,et.al
【作者單位】廈門(mén)理工大學(xué)(Xiamen University of Technology)
【文獻(xiàn)中引用產(chǎn)品】
微囊藻毒素LR(MC-LR)elisa試劑盒
【關(guān)鍵詞】Microcystin-leucine-arginine,Environmentally relevant concentrations,Acorus calamus Linnaeus,Nitrogen metabolism
【DOI】https://doi.org/10.1007/s00128-019-02597-y
【影響因子(IF)】2.9
【出版期刊】《Bulletin of Environmental Contamination and Toxicology》
【產(chǎn)品原文引用】
Acorus calamus L. plants with 10–12 cm long apical shoots were purchased from a local nursery (Xiamen, Fujian, China). They were then washed clean with deionized water and flushed three times with sterile water. Finally, each plant was inserted into a hole in a foam board with one foam board bearing three A. calamus L. shoots. The boards were then separately placed in a glass cylinder with 2L of 1/4 Hoagland nutritive solution (pH 6.5) and pre-cultured for 5 days. Subsequently, the nutritive solution was replaced with 1/4 Hoagland nutritive solution containing different concentrations of MC–LR (0 μg/L as the control group, and 1.0, 9.9, and 29.8 μg/L as treatment groups). The pure MC-LR (purity≥96%) was purchased from Alexis Biochemicals (Lufelfingen, Switzerland). The MC–LR concentrations in the nutritive solution were determined using enzyme linked immunoassay kit (detection limit 20ng/L) (ShangHai HengYuan Biological Technology Co., Ltd China).
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